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804g bladder carcinoma cell line  (ViaCyte Inc)

 
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    ViaCyte Inc 804g bladder carcinoma cell line
    Glucose- and calcium-mediated activation of ATP synthase-dependent respiration in human islets. Human islets bound to <t>804G</t> matrix were incubated in KRBH ( A ) or a KRBH buffer lacking Ca 2+ supplemented with 0.4 m m EGTA ( B ; Ca 2+ -free). For each condition the mean ± S.E. n = 3 from the same donor is shown. Total islet protein varied between wells (4–6 μg). Because of these variations the results are expressed relative to the respiratory rate before glucose stimulation. ATP synthase-dependent ( dep , C ) and -independent respiration ( D ) was quantified as described under “Experimental Procedures.” The results are the mean ± S.E. ( n = 6) obtained from 2 donors. *, p < 0.01; **, p < 0.001; ns , not significant. R , rotenone (1 μ m ); AA , antimycin A (1 μg/ml); O , oligomycin (2.5 μg/ml); glc , glucose. E , preventing calcium signaling blocks glucose-induced insulin secretion. Insulin secretion from human islets was determined in KRBH or the same buffer lacking Ca 2+ in either 1 m m ( gray bars ) or 16.7 m m glucose ( black bars ). Shown is the average ±S.E. n = 4 result with islets from a single donor (*, p < 0.05).
    804g Bladder Carcinoma Cell Line, supplied by ViaCyte Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/804g bladder carcinoma cell line/product/ViaCyte Inc
    Average 90 stars, based on 1 article reviews
    804g bladder carcinoma cell line - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "Calcium Co-regulates Oxidative Metabolism and ATP Synthase-dependent Respiration in Pancreatic Beta Cells"

    Article Title: Calcium Co-regulates Oxidative Metabolism and ATP Synthase-dependent Respiration in Pancreatic Beta Cells

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M113.513184

    Glucose- and calcium-mediated activation of ATP synthase-dependent respiration in human islets. Human islets bound to 804G matrix were incubated in KRBH ( A ) or a KRBH buffer lacking Ca 2+ supplemented with 0.4 m m EGTA ( B ; Ca 2+ -free). For each condition the mean ± S.E. n = 3 from the same donor is shown. Total islet protein varied between wells (4–6 μg). Because of these variations the results are expressed relative to the respiratory rate before glucose stimulation. ATP synthase-dependent ( dep , C ) and -independent respiration ( D ) was quantified as described under “Experimental Procedures.” The results are the mean ± S.E. ( n = 6) obtained from 2 donors. *, p < 0.01; **, p < 0.001; ns , not significant. R , rotenone (1 μ m ); AA , antimycin A (1 μg/ml); O , oligomycin (2.5 μg/ml); glc , glucose. E , preventing calcium signaling blocks glucose-induced insulin secretion. Insulin secretion from human islets was determined in KRBH or the same buffer lacking Ca 2+ in either 1 m m ( gray bars ) or 16.7 m m glucose ( black bars ). Shown is the average ±S.E. n = 4 result with islets from a single donor (*, p < 0.05).
    Figure Legend Snippet: Glucose- and calcium-mediated activation of ATP synthase-dependent respiration in human islets. Human islets bound to 804G matrix were incubated in KRBH ( A ) or a KRBH buffer lacking Ca 2+ supplemented with 0.4 m m EGTA ( B ; Ca 2+ -free). For each condition the mean ± S.E. n = 3 from the same donor is shown. Total islet protein varied between wells (4–6 μg). Because of these variations the results are expressed relative to the respiratory rate before glucose stimulation. ATP synthase-dependent ( dep , C ) and -independent respiration ( D ) was quantified as described under “Experimental Procedures.” The results are the mean ± S.E. ( n = 6) obtained from 2 donors. *, p < 0.01; **, p < 0.001; ns , not significant. R , rotenone (1 μ m ); AA , antimycin A (1 μg/ml); O , oligomycin (2.5 μg/ml); glc , glucose. E , preventing calcium signaling blocks glucose-induced insulin secretion. Insulin secretion from human islets was determined in KRBH or the same buffer lacking Ca 2+ in either 1 m m ( gray bars ) or 16.7 m m glucose ( black bars ). Shown is the average ±S.E. n = 4 result with islets from a single donor (*, p < 0.05).

    Techniques Used: Activation Assay, Incubation



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    804g bladder carcinoma cell line  (ViaCyte Inc)
    90
    ViaCyte Inc 804g bladder carcinoma cell line
    Glucose- and calcium-mediated activation of ATP synthase-dependent respiration in human islets. Human islets bound to <t>804G</t> matrix were incubated in KRBH ( A ) or a KRBH buffer lacking Ca 2+ supplemented with 0.4 m m EGTA ( B ; Ca 2+ -free). For each condition the mean ± S.E. n = 3 from the same donor is shown. Total islet protein varied between wells (4–6 μg). Because of these variations the results are expressed relative to the respiratory rate before glucose stimulation. ATP synthase-dependent ( dep , C ) and -independent respiration ( D ) was quantified as described under “Experimental Procedures.” The results are the mean ± S.E. ( n = 6) obtained from 2 donors. *, p < 0.01; **, p < 0.001; ns , not significant. R , rotenone (1 μ m ); AA , antimycin A (1 μg/ml); O , oligomycin (2.5 μg/ml); glc , glucose. E , preventing calcium signaling blocks glucose-induced insulin secretion. Insulin secretion from human islets was determined in KRBH or the same buffer lacking Ca 2+ in either 1 m m ( gray bars ) or 16.7 m m glucose ( black bars ). Shown is the average ±S.E. n = 4 result with islets from a single donor (*, p < 0.05).
    804g Bladder Carcinoma Cell Line, supplied by ViaCyte Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/804g bladder carcinoma cell line/product/ViaCyte Inc
    Average 90 stars, based on 1 article reviews
    804g bladder carcinoma cell line - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Glucose- and calcium-mediated activation of ATP synthase-dependent respiration in human islets. Human islets bound to 804G matrix were incubated in KRBH ( A ) or a KRBH buffer lacking Ca 2+ supplemented with 0.4 m m EGTA ( B ; Ca 2+ -free). For each condition the mean ± S.E. n = 3 from the same donor is shown. Total islet protein varied between wells (4–6 μg). Because of these variations the results are expressed relative to the respiratory rate before glucose stimulation. ATP synthase-dependent ( dep , C ) and -independent respiration ( D ) was quantified as described under “Experimental Procedures.” The results are the mean ± S.E. ( n = 6) obtained from 2 donors. *, p < 0.01; **, p < 0.001; ns , not significant. R , rotenone (1 μ m ); AA , antimycin A (1 μg/ml); O , oligomycin (2.5 μg/ml); glc , glucose. E , preventing calcium signaling blocks glucose-induced insulin secretion. Insulin secretion from human islets was determined in KRBH or the same buffer lacking Ca 2+ in either 1 m m ( gray bars ) or 16.7 m m glucose ( black bars ). Shown is the average ±S.E. n = 4 result with islets from a single donor (*, p < 0.05).

    Journal: The Journal of Biological Chemistry

    Article Title: Calcium Co-regulates Oxidative Metabolism and ATP Synthase-dependent Respiration in Pancreatic Beta Cells

    doi: 10.1074/jbc.M113.513184

    Figure Lengend Snippet: Glucose- and calcium-mediated activation of ATP synthase-dependent respiration in human islets. Human islets bound to 804G matrix were incubated in KRBH ( A ) or a KRBH buffer lacking Ca 2+ supplemented with 0.4 m m EGTA ( B ; Ca 2+ -free). For each condition the mean ± S.E. n = 3 from the same donor is shown. Total islet protein varied between wells (4–6 μg). Because of these variations the results are expressed relative to the respiratory rate before glucose stimulation. ATP synthase-dependent ( dep , C ) and -independent respiration ( D ) was quantified as described under “Experimental Procedures.” The results are the mean ± S.E. ( n = 6) obtained from 2 donors. *, p < 0.01; **, p < 0.001; ns , not significant. R , rotenone (1 μ m ); AA , antimycin A (1 μg/ml); O , oligomycin (2.5 μg/ml); glc , glucose. E , preventing calcium signaling blocks glucose-induced insulin secretion. Insulin secretion from human islets was determined in KRBH or the same buffer lacking Ca 2+ in either 1 m m ( gray bars ) or 16.7 m m glucose ( black bars ). Shown is the average ±S.E. n = 4 result with islets from a single donor (*, p < 0.05).

    Article Snippet: The 804G bladder carcinoma cell line ( ) and protocols to isolate the matrix were obtained from Viacyte (San Diego, CA).

    Techniques: Activation Assay, Incubation